Product Overview
The Bruker CONFOCHECK Protein Analyzer is an elite spectroscopic workstation designed specifically for biopharmaceutical research, formulation development, and quality control. Unlike traditional methods that require sample modification, the CONFOCHECK allows for the holistic investigation of soluble and membrane-bound proteins in their intended natural environment, using original biological buffers and formulations. By analyzing the characteristic Amide I and II bands, the system provides high-sensitivity detection of conformational changes, even in the presence of complex excipients and detergents.
The system's modularity is defined by its application-specific sampling accessories, which facilitate a wide range of analytical tasks without instrument reconfiguration. The AquaSpec flow-through cell enables high-throughput determination of secondary structure and concentration, while the BioATR II unit allows for automated, temperature-controlled studies of protein dynamics and folding processes from 4°C to 95°C. Integrated with fully validated OPUS software, the CONFOCHECK delivers 21 CFR Part 11 compliant data, ensuring that structural insights are both scientifically robust and meeting the highest regulatory standards.
Product Demonstration
Key Features
- Native State Analysis: Directly measure proteins in aqueous solutions (H₂O) without the need for deuterated water (D2O) or sample dilution.
- Secondary Structure Prediction: Quantify the absolute percentage of alpha-helix, beta-sheet, and random coil elements within seconds via internal calibrations.
- BioATR II Technology: Perform automated temperature ramps to determine protein melting points and monitor aggregation or fibrillation kinetics in real-time.
- Broad Dynamic Range: Achieve high-precision results for protein concentrations ranging from 0.03 µg/µl to over 100 µg/µl.
- Low Sample Consumption: Requires as little as 20 µg/µl of sample material, making it ideal for high-value biologics and limited research specimens.
- Dual-Channel Modularity: Utilize an independent analysis channel (Transit Platinum-ATR) for parallel measurement of solid samples, lyophilizates, or packaging materials.
- Full Regulatory Readiness: Comprehensive compliance with 21 CFR Part 11, cGMP, and international pharmacopeia standards with automated validation routines.
Specifications
| Spectral Range | 8,000 – 850 cm-1 (Liquids); 8,000 – 350 cm-1 (Solids) |
| Protein Concentration | 0.03 µg/µl to over 100 µg/µl |
| Required Sample Volume | > 20 µl |
| Temperature Range | 4 – 95 ºC (with BioATR II) |
| Acquisition Time | Typically 1 minute (AquaSpec) / 10 seconds (Solids) |
| Detector | High-sensitivity PV-MCT for maximum linearity |
| Compliance | 21 CFR Part 11, cGMP, GLP, and International Pharmacopeia |