Kaplan Scientific
Educational Series

Spectroscopy 101

Welcome to the fascinating world of molecular spectroscopy. Learn how light unlocks the secrets of matter.

What is Spectroscopy?

Ideally, spectroscopy is the study of the interaction between matter and electromagnetic radiation. Historically, spectroscopy originated through the study of visible light dispersed according to its wavelength, by a prism.

In simple terms: Spectroscopy is the science of using light to measure chemicals.

By analyzing the light that is absorbed, emitted, or scattered by a sample, we can determine:

  • Identity: What is it?
  • Concentration: How much is there?
  • Structure: How are the atoms arranged?
Electromagnetic Spectrum showing wavelengths from radio waves to gamma raysLight Refraction: White Light & Prism showing dispersion into rainbow spectrum

Core Concepts

Light & Matter

Spectroscopy studies the interaction between light (electromagnetic radiation) and matter. Each chemical substance interacts with light in a unique way.

The Spectrum

When light interacts with a sample, significantly more information is returned than just color. We get a "spectrum" - a plot of intensity vs. wavelength.

Identification

Like a human fingerprint, every molecular structure produces a unique spectral pattern. By matching this pattern against a library, we identify the substance.

Quantification

The intensity of the signal is directly proportional to the concentration (Beer-Lambert Law). This allows us to measure "how much" of a substance is present.

Video Lessons

Educational content from Khan Academy®

FTIR Spectroscopy Lessons

Infrared Spectroscopy Overview

IR Spectroscopy Basics

Introduction to Infrared Spectroscopy

IR Spectroscopy Fundamentals

IR Spectrum Analysis

Interpreting IR Spectra

Functional Groups in IR

IR Spectroscopy Practice

Advanced IR Techniques

IR Spectroscopy Applications

IR Problem Solving

Content provided by Khan Academy®
All KHAN ACADEMY® content is available for free at www.khanacademy.org

Major Techniques

The three pillars of vibrational spectroscopy

FT-IR Spectroscopy

Fourier Transform Infrared Spectroscopy

The "fingerprinting" technique of the chemical world. It identifies chemical bonds in a molecule by producing an infrared absorption spectrum.

Applications:
Quality ControlMaterial IdentificationForensicsPharma Analysis
Deep Dive

Raman Spectroscopy

Raman Scattering Technique

A non-destructive scattering technique that provides detailed information about chemical structure, phase and polymorphy, crystallinity and molecular interactions.

Applications:
PharmaceuticalsGemologyArt ConservationSemiconductors
Deep Dive

NIR Spectroscopy

Near-Infrared Spectroscopy

Uses the near-infrared region of the electromagnetic spectrum. It is widely used for quantitative analysis and process control.

Applications:
AgricultureFood QualityChemical ProcessPetrochemicals
Deep Dive

Ready to apply this knowledge?

Explore our Products and Application Notes to see real-world examples.

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